Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Margaret Ruzicka
Date created: 2008-08-28 11:00:00
Date modified: 2015-05-08 01:09:45
Replication Proteins
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K125510_sequence (Version 1) |
Description
Plasmids containing oriV and the corresponding Rep proteins (from RSF1010) are autonomously replicated in a broad range of hosts. It has been reported that RSF1010 derived plasmids containing oriV and the Rep proteins are stably maintained in Pseudomonas (Bagdasarian 1981), Caulobacter (Umelo-Njaka et al. 2001), Erwinia, Serratia (Leemans 1987), and several cyanobacteria strains (Mermet-Bouvier 1993) including Synechocystis PCC6803 and PCC6714 and Synechococcus PCC7942 and PCC6301.Notes
This part will be constructed with the origin of vegetative replication derived from an RSF1010 plasmid.Source
This part is taken from the RSF1010 derived plasmid pRL1383a. A description of this plasmid can be found at the NCBI database at accession number AF403426.| Sequence Annotation | Location | Component / Role(s) |
| I14032 B0030 RepB' Promoter[EFRepAC] [EFRepAC] Native Terminator | 1,37 46,60 67,1038 1028,1079 1099,3226 3353,3385 | engineered_region feature/BioBrick feature/BioBrick engineered_region CDS feature/cds feature/promoter promoter feature/cds CDS stem_loop feature/stem_loop |