BBa_K125530

BBa_K125530 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K125530

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Margaret Ruzicka

Date created: 2008-10-25 11:00:00

Date modified: 2015-05-08 01:09:45

aadA gene with lacIQ promoter and RBS

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K125530_sequence (Version 1)

Description

The aadA gene confers resistance to Spectinomycin and Streptomycin. The [[Part:BBa_I14032|lacIQ promoter]] and an [[Part:BBa_B0034|RBS] were added. This part is functional without a terminator.

Notes

This part will be tested on varying concentrations of antibiotics.

Source

This part was amplified from pRL1383a, an RSF1010 derived plasmid.

Components

Access	Instance	Definition
public	BBa_I14032	P(Lac) IQ
public	BBa_B0034	BBa_B0034
public	BBa_K125520	aada

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I14032	1,37	P(Lac) IQ
BBa_B0034	46,57	BBa_B0034
BBa_K125520	64,855	aada

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K125530/1

Member of these Collections

• iGEM Parts Registry