BBa_K1260000

BBa_K1260000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1260000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhengfeng Liu
Date created: 2014-04-24 11:00:00
Date modified: 2015-05-08 01:09:45

AFB1 ScFv with a his-tag, a SH3 ligand and a signal peptide



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1260000_sequence (Version 1)

Description

AFB1 ScFv with a his-tag, a SH3 ligand and a signal peptide. The signal peptide is at the beginning of the sequence. With the part, you can let your E.coli produce antibody to target the aflatoxin B1.

Notes

We have improved the codons of the coding sequence.

Source

The part is constructed artificially.

Sequence Annotation Location Component / Role(s)
start
ssDsbA
AFB1-ScFv
linker 1
6*his-tag
linker 2
SH3 Ligand
stop
1,3
1,69
88,819
70,87
820,837
847,891
891,924
925,930
start_codon feature/start
feature/misc sequence_feature
CDS feature/protein
feature/misc sequence_feature
feature/tag tag
sequence_feature feature/misc
sequence_feature feature/misc
stop_codon feature/stop
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1260000/1