Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Burak YILMAZ
Date created: 2008-11-08 12:00:00
Date modified: 2015-05-08 01:09:46
LamB_CP-metal binding domain in LamB-153 residue
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K129005_sequence (Version 1) |
Description
LamB tolerates insertions of long heterologous peptides at a permissive loop (between structural codons 153 and 154) exposed to the external medium without a loss of function.CP (Gly-Cys-Gly-Cys-Pro-Cys-Gly-Cys-Gly), employed in LamB and called LamB_CP. Cp was previously found as
cysteine and histidine residues for Cd21 binding (29). CP was
further characterized and employed for display on the E. coli
surface.
Notes
To make HP_LamB GATCCAGCTGGTCATCATCCACACGGTGCT is inserted in LamB protein at 153Source
GATCCAGCAGGCTGCGGTTGTCCATGCGGTTGTGGCGCT encodes the N-Ala-Gly-Cys-Gly-Cys-Pro-Cys-Gly-Cys-Gly-Ala-C sequenceinserted in LamB-153 residue because permissive loop (between structural codons 153and 154) can be exposed to the external medium without a loss of function.