LuxOU

BBa_K131016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K131016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Thane Kubik

Date created: 2008-09-27 11:00:00

Date modified: 2015-05-08 01:09:47

LuxOU from Vibrio harveyi

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K131016_sequence (Version 1)

Description

Vibrio harveyi repressor protein LuxO and phosphorelay protein LuxU genes. LuxO and LuxU are regulators of luminescence in Vibrio harveyi.

Notes

LuxO and LuxU are found within the operon, therefore we have decided to keep these genes together. The natural RBS was also kept.

Source

GenBank Accession# L26221
LuxO Protein ID# AAD12736.1
LuxU Protein ID# AAD12737.2

Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS	5,12	ribosome_entry_site, feature/rbs
LuxO	31,1392	CDS, feature/cds
Start for LuxO	31,33	feature/start, start_codon
Stop for LuxO	1390,1392	stop_codon, feature/stop
LuxU	1389,1733	CDS, feature/cds
Start for LuxU	1389,1391	feature/start, start_codon
Stop for LuxU	1731,1733	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K131016/1

Member of these Collections

• //function/cellsignalling
• //function/regulation/transcriptional
• iGEM Parts Registry