LuxO D47E

BBa_K131022 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K131022

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Thane Kubik

Date created: 2008-10-27 12:00:00

Date modified: 2015-05-08 01:09:47

LuxO D47E, Vibrio harveyi

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K131022_sequence (Version 1)

Description

LuxO D47E, Vibrio harveyi is a constitutively active mutant of LuxO

Notes

The plasmid containing LuxOD47E (pJAF822) was obtained from Bassler lab (Freeman and Bassler, 1999).

Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.

Source

>gi|156972381:c2983472-2982069 Vibrio harveyi ATCC BAA-1116 chromosome I, complete sequence
Nucleotide number 141 has been mutated from T to G, thus bases 139-141 have been changed from GAT (Aspartic acid, D) to GAG (Glutamine, E).
Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
D47E	139,141	feature/mutation, sequence_alteration
Start	1,3	start_codon, feature/start
Stop	1360,1362	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K131022/1

Member of these Collections

• //cds/transcriptionalregulator/activator
• //function/cellsignalling
• iGEM Parts Registry