BBa_K1313001

BBa_K1313001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1313001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eddie Cano

Date created: 2014-10-10 11:00:00

Date modified: 2015-05-08 01:09:47

Oxoacyl Reductase

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1313001_sequence (Version 1)

Description

7-ketocholesterol is the result of the oxidation that exists in the bloodstream. 7-keto group is being reduced by Oxoacyl Reductase into 7-OH. The identity of this enzyme has not yet veryfied. This nucleotidic sequence was based on Jacques Mathieu studies.He identified gene expression analysis using microarrays and in silico amino acidic predictions.

Notes

Codon optimization for a better expression on E.coli.

Source

ATGAAACGCCTGGTCTGTGTTCTGCTGGTCTGCTCGTCTGCTGTTGCTCCGCTGCATCATAGCCTGGTTGATCAC
CCGTCTGCCCATCCGGGTCATGGCGGTGGCCCTGTTAGTGCGGGCGTCGCCCTGGTGACCGGTGGCTCCCGTGGC
GTTGGTAAAGGCGTCGCAACGGCACTGGGTGCGGCGGGTTGGACCGTGTATGTTACGGGTCGTGGCCCGATGACC
GAAGATTGCCCGCTGAGTGCATCCGCTCGTACCGTTACGGGTGCGGGTGGCACCGGTATTGCAGTCGCCTGTGAC
CACCGTGAAGATGCAGACGTGCGTGCACTGTTTGCTCGCATCAGTGATGAACAGGCCGGTCGCCTGGACCTGCTG
GTTAACAATGTCTGGGCAGCTCCGAAAGGCTTTGGTGGCTTCAGCACCCCGTTCTGGGAACGTCCGCTGGATGAC
TGGGATTCTCTGATTGGTGTTGGCCTGCGTGCGCATTATGTGGCCTCAGTTGAAGCAGCACGTCTGATGGTGCCG
CGTCGCTCGGGTCTGATTGCGAACATCAGCTCTTTTGGTACCCGCGGCCATCTGCACTCAGTGCTGTACGGCATG
TCGAAAGCAGGCCTGGATAAAATGGCTAGCGACATGGCAGCTGAACTGGCCCCGCACGATGTCCGTGCAGTGAGT
CTGTGGCCGGGCATTGTGCGCACCGAAATGATCGTTGCGTCCGGTCTGGAAACGATCGCCGGCTTTAGCGTTGCA
GATGCTGAATCTCCGGAATTTATTGGTGAAGTCATCTGCGCACTGACCGCCGACCCGACGCTGCCGCAGCGTTCT
GGTCATACCTTCGTCACGGCAGAACTGGCTGTGGAATACGGTATTACCGATACGCAAGGTCGTACCCCGCCGAGC
CACCGTGAACCGTTTGGTGGCGGTCCGCTGTTCAATATCACG

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1313001/1

Member of these Collections

• //cds/enzyme
• iGEM Parts Registry