BBa_K1316003

BBa_K1316003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1316003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joan Cortada Garcia
Date created: 2014-08-23 11:00:00
Date modified: 2015-05-08 01:09:50

yqjF promoter coupled to mKate2 reporter gene



Types
DnaRegion

Roles
engineered_region

Measurement

Sequences BBa_K1316003_sequence (Version 1)

Description

Promoter of the yqjF gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli.
This construct is, then, designed to be able to detect and quantify the presence of compunds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB.

Notes

The yqjF promoter carries 4 point mutations to improve its response in front of 2,4,6-TNT and 2,4-DNT

Source

Combination of K1316002 with K1316001

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1316003/1