Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joan Cortada Garcia
Date created: 2014-08-23 11:00:00
Date modified: 2015-05-08 01:09:50
yqjF promoter coupled to mKate2 reporter gene
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_K1316003_sequence (Version 1) |
Description
Promoter of the yqjF gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli.This construct is, then, designed to be able to detect and quantify the presence of compunds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB.
Notes
The yqjF promoter carries 4 point mutations to improve its response in front of 2,4,6-TNT and 2,4-DNTSource
Combination of K1316002 with K1316001| Access | Instance | Definition |
| public public | BBa_K1316002 BBa_K1316001 | BBa_K1316002 BBa_K1316001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1316002 BBa_K1316001 | 1,271 280,1099 | BBa_K1316002 BBa_K1316001 |