BBa_K1316016

BBa_K1316016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1316016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Joan Cortada Garcia

Date created: 2014-09-28 11:00:00

Date modified: 2015-05-08 01:09:50

constitutively expressed eGFP under the control of J23110 Anderson promoter

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1316016_sequence (Version 1)

Description

Constitutively expressed Enhanced Green Fluorescen Protein (eGFP). eGFP is more intense than normal GFP.
The purpose of this construct is to be able to easily detect cells attached to a surface, therefore detecting biofilm formation cells. For our project that would be used for detecting curli-forming cells.

Notes

eGFP was desired to be constitutively expressed.
The sequence was screened for illegal iGEM restriction sites

Source

eGFP was PCRed out a conventional plasmid containing the coding sequence for this reporter gene.
The constitutive promoter is the iGEM J23110 Anderson promoter

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
J23110 Anderson promoter	1,35	promoter, feature/promoter
rbs	45,52	ribosome_entry_site, feature/rbs
EGFP	60,737	CDS, feature/cds
stop	735,737	feature/stop, stop_codon
start	60,62	start_codon, feature/start

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1316016/1

Member of these Collections

• iGEM Parts Registry