Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mounir Benkoulouche
Date created: 2014-10-06 11:00:00
Date modified: 2016-02-10 11:29:51
CDS of silk-like protein (SLP)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1317002_sequence (Version 1) |
Description
This part is the coding sequence for the silk like polypeptide. This sequence was assembled from a consensus of the MaSp1 from spiders. This protein, in spiders, allows resistance and elasticity used for spinning the webs.The synthetic gene encodes a synthetic protein "silk-like". The repeated aminoacids allow to retrieve these properties of resistance, resilience and elasticity, but with a minimal pattern of the original protein, which is more suited for downstream applications. It can be used to produce wire presenting these properties after wet-spinning.
Notes
The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for the assembling.Source
major ampullate spidroin 1 (MaSp1) gene from spiders| Sequence Annotation | Location | Component / Role(s) |
| Start Codon Stop Codon CDS Aromatic (dosage) ChewBack NheI | 6,8 330,332 6,332 9,11 318,329 312,317 | start_codon feature/start stop_codon feature/stop CDS feature/cds feature/tag tag feature/misc sequence_feature feature/BioBrick engineered_region |