REACh2

BBa_K1319002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1319002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Osthege, Florian Gohr
Date created: 2014-09-29 11:00:00
Date modified: 2015-05-08 01:09:50

RFC[25]-compatible dark quencher based on K1319000 (E0030)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1319002_sequence (Version 1)

Description

This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP).

Three mutations were introduced that eliminated fluorescence:
  • L90I
  • Y145W
  • H148R


Notes

Point mutations were introduced, starting from K1319000 (RFC25-YFP) as the template.

Source

The part is derived from K1319000.

Sequence Annotation Location Component / Role(s)
REACh2
1,711
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1319002/1