BBa_K1321004

BBa_K1321004 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K1321004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xenia Spencer-Milnes
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:09:51

N-terminal rfc10_C-terminal rfc25 assembly



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_K1321004_sequence (Version 1)

Description

This part has not been cloned, it contains the sequence which is made at the beginning of an rfc25 format coding region when the prefix is digested with SpeI and ligated to an XbaI site in an rfc10 backbone. This allows entry of rfc25 format parts as C-terminal fusions with N-terminal rfc10 parts as composite parts correctly.

t ACTAGA TGGCCGGC

Notes

Functional 'Scar' site

Source

Functional 'Scar' site

igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1321004/1