BBa_K1321300

BBa_K1321300 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1321300

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Michael Florea

Date created: 2014-10-07 11:00:00

Date modified: 2015-05-08 01:09:52

pSEVA331-Bb - pSEVA331 with biobrick prefix and suffix

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_K1321300_sequence (Version 1)

Description

This is pSEVA331 converted into a biobrick compatible format by substituting the original multiple cloning site for biobrick prefix and suffix. pSEVA331 is a broad host range plasmid, capable of replication in E.coli and several other organisms. It is capable of replication in the cellulose producing bacterium Gluconacetobacter xylinum. We have used it as our standard backbone for G.xylinum genetic engineering.

Notes

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Source

The original pSEVA331 backbone comes from the SEVA (Standard European Vector Architecture) collection.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Biobrick suffix	1,22	sequence_feature, feature/misc
Biobrick prefix	2872,2893	feature/misc, sequence_feature
Chloramphenicol acetyltransferase	271,930	feature/protein, CDS
OriT	954,1189	sequence_feature, feature/misc
Rep	1198,1860	sequence_feature, feature/misc

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1321300/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors