BBa_K1321333

BBa_K1321333 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1321333
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura de Arroyo Garcia
Date created: 2014-10-07 11:00:00
Date modified: 2015-06-17 12:27:41

pSB-AraC-pBAD



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1321333_sequence (Version 1)

Description

This regulatory part is made up of the Arabinose-Inducible promoter, pBAD, and its transcriptional inhibitor/activator, AraC. AraC inhibits gene expression in the absence of Arabinose by binding to the operator units present within the AraC and pBAD promoter sequences, and producing a DNA loop that prevents the RNA polymerase and CAP from binding and initiating transcription. When Arabinose is supplied, this binds to the AraC dimer and releases it from the operator site found in the pBAD promoter, at the same time it enhances transcription by allowing binding to the enhancer sites upstream.

Source

This part was cloned out of existing templates (such as BBa_K325218, BBa_325219, all of which contain sfGFP downstream of the pBAD promoter)

Sequence Annotation Location Component / Role(s)
AraC
PC
pBAD
1,1041
1042,1115
1116,1210
feature/cds CDS
feature/promoter promoter
feature/promoter promoter
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1321333/1