Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jarrod Shilts
Date created: 2014-10-15 11:00:00
Date modified: 2015-05-08 01:09:53
Santalene Synthase
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1322231_sequence (Version 1) |
Description
BBa_K1322231 is a codon-optimized biobrick part encoding the gene for alpha-santalene synthase (EC 4.2.3.82). The enzyme catalyze the conversion of the common isoprenoid intermediate farnesyl pyrophosphate (FPP) into the sesquiterpene (+)-alpha-santelene in a single step. Traces of (-)-beta-santalene and bergamontene have previously been shown to be produced by this enzyme as well.The gene, derived from a relative of the exotic sandalwood tree, has been demonstrated to produce functional terpene product in both yeast (Scalcinati et al 2012) and E. coli (data pending). This is possible due to several endogenous pathways that produce FPP as an intermediate, including the MEV and MEP pathways,
In addition to being a prized fragrance, with what is often described as a warm, sweet woody scent, the sandalwood oil has been investigated for a number of other practical applications, including as a chemoprotective against carcinogenesis (Banaerjee, Ecavade, and Rao 1993) and inhibitors of viral reproduction (Koch et al 2008).
References:
Scalcinati et al.: Combined metabolic engineering of precursor and co-factor supply to increase α-santalene production by Saccharomyces cerevisiae. Microbial Cell Factories 2012 11:117.
Banerjee, Ecavade and Rao: Modulatory influence of sandalwood oil on mouse hepatic glutathione S-transferase activity and acid soluble sulphydryl level. Cancer Letters, 68 (1993) 105 - 109
Koch et al: Inhibitory effect of essential oils against herpes simplex virus type 2. Phytomedicine 2008;15(1-2):71-8.
Notes
Two sites on the original gene sequence had to be changed by selected sense mutation codons in order to make the part RFC10 compatible.Source
The sequence comes from genomic DNA taken from the santalum genus of plant. It was codon optimized for expression in Saccharomyces cerevisiae. The part also contains a yeast consensus sequence before the start codon, a C-terminal strep tag, and biobrick prefixes and suffixes.| Sequence Annotation | Location | Component / Role(s) |
| Yeast Consensus Sequence Santalene Synthase Strep Tag | 7,13 14,1667 1668,1692 | feature/rbs ribosome_entry_site feature/cds CDS tag feature/tag |