BBa_K1329003

BBa_K1329003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1329003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniela Geist

Date created: 2014-10-06 11:00:00

Date modified: 2015-05-08 01:09:54

Hag-D2-Strep

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1329003_sequence (Version 1)

Description

In order to build up flagella usable as protein scaffold we wanted to design a system which makes it possible to switch a high variety of functional modules. For that purpose we designed a flagellin construct containing a Strep-Tag inserted into the D2 domain of Salmonella typhimurium on top of the Bacillus Subtilis flagellin as a linker region.
By exposure of the Strep-Tag to the outside of the flagellum it would be possible to connect Streptavidin-hybridized fusion proteins of various functionalities to the flagellum as scaffold increasing the epitope density.

Notes

GSGSGS-Linkers as flanks of the Strep-Tag for better exposure to the outside

Source

Genome of Bacillus Subtilis
Genome of Salmonella Typhimurium
Protein sequence of the Strep-Tag was found via pdb.org

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1329003/1

Member of these Collections

• iGEM Parts Registry