BBa_K133046

BBa_K133046 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K133046
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ana Lasic
Date created: 2008-10-29 12:00:00
Date modified: 2015-05-08 01:09:55

CF215



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K133046_sequence (Version 1)

    Description

    This is a part that is composed of C-terminal end of chimeric flagellin (BBa_K133016). We used aminoacids from 215th aminoacid on.

    Chimeric flagellin is composed of C- and N-terminal part of E.coli flagellin and of variable region of H.pylori. The E.coli parts are responsible for binding and activation of cell membrane receptor TLR5 to initiate immune response, while H.pylori variable region was used to array the immune response against H.pylori.

    This part was used in combination with CF213 - chimeric flagellin (BBa_K133010). Between the CF213 and CF215 we have put H.pylori antigens in order to array the immune response against H.pylori.

    Notes

    It was important to split the chimeric flagellin in the variable region to assure that the antigen will be in the right place and not to interrupt the 3d structure of the protein.

    Source

    We used polimerase chain reaction from chimeric flagellin (BBa_K133016).

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#topLevel
    BBa_K133046/1
     

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