BBa_K1333201

BBa_K1333201 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1333201
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yanwen Xie
Date created: 2014-09-08 11:00:00
Date modified: 2015-05-08 01:09:58

lambda cI-LGF2(D78A)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1333201_sequence (Version 1)

Description

Fuse lambda cI with LGF2 mutated from Asp to Ala at site 78. As one of the components in Bacterial Two-hybrid System, it causes a less interaction with GAL11P compared to the nomalized WT.

Notes

Mutation of GAC to GCC LGF2 at site 78.

Source

From Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit.

Sequence Annotation Location Component / Role(s)
lambda cI
LGF2(D78A)
ATG start codon
TGA stop codon
1,711
721,840
1,3
841,843
CDS feature/protein
CDS feature/protein
feature/start start_codon
feature/stop stop_codon
igem#experience
Works
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1333201/1