BBa_K1335001

BBa_K1335001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1335001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Luiza Niyazmetova

Date created: 2014-10-15 11:00:00

Date modified: 2015-05-08 01:09:58

Vh p53 + Ig hinge + his tag + HlyA

Details

• Types: DnaRegion
• Roles: engineered_region; Composite

Description

Vh p53 is responsible for the production of nanobodies that have an affinity to p53 protein: this interaction can be used in cancer detection.

Notes

GTTTCTTCGAATTCGCGGCCGCTTCTAGatggaggtgcagctggtggagtctgggggaggcttggtgcagcctggggggtctctgagactctcctgtgcagcctctggattcaccttcgatggtttccggatgtattgggtccgtcaggcaccggggaaggggctcgagtgggtctcggctattaatacgagtggtcgcaccacatactatgcagactccgtgaagggccgattcatcatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgaaatctgaggacacggccgtgtattactgcgcaaaagatggaggatactatagtcgtatgtactactttgggtactggggccaggggacccaggtcaccgtctcctcagcctcgggggccgcgtcgacgtccggcggtccgaagccttccactccgcccgggtcttcccgtatgaaacagctggaagacaaagtagaggagctccttagcaagaactaccatctcgaaaacgaggtagctcgtctgaaaaagcttgttggtgaacgtggtggtgcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctcaccatcaccatcaccatgcgtcgacgcccgggggtgcgccggtgccgtatccggatccgctggaaccggccggggaaaattctcttgctaaaaatgtattatccggtggaaaaggtaatgacaagttgtacggcagtgagggagcagacctgcttgatggcggagaagggaatgatcttctgaaaggtggatatggtaatgatatttatcgttatctttcaggatatggccatcatattattgacgatgaaggggggaaagacgataaactcagtttagctgatatagatttccgggacgttgcctttaagcgagaagggaatgacctcattatgtataaagctgaaggtaatgttctttctattggccacaaaaatggtattacatttaaaaactggtttgaaaaagagtcagatgatctctctaatcatcagatagagcagatttttgataaagacggcagggtaatcacaccagattctcttaaaaaagcatttgaatatcagcagagtaataacaaggtaagttatgtgtatggacatgatgcatcaacttatgggagccaggacaatcttaatccattaattaatgaaatcagcaaaatcatttcagctgccggtaacttcgatgttaaggaggaaagatctgccgcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataactttgacagcatcagcataaGTTTCTTCCTGCAGCGGCCGCTACTAGTA
The gene is not in standard pSB1C3 plasmid but in pUC57 vector, but it contains standard prefix and suffix.

Source

Naturally, nanobodies are present in camelids family, however we decided to use the approach of bacterial production. The gene was synthesized by GenScript company.

Other Properties

• igem#sampleStatus: No part sequence
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1335001/1

Member of these Collections

• iGEM Parts Registry