BBa_K1343005

BBa_K1343005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1343005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon

Date created: 2014-09-26 11:00:00

Date modified: 2015-05-08 01:09:59

PCI-tetR

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1343005_sequence (Version 1)

Description

tetR repressor protein under lambda phage CI regulated promoter

Notes

To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other

Source

Placeholder

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0051:promoter (lambda cI regulated)	1,49	feature/promoter, promoter
Barcode Label 6	50,71	sequence_feature, feature/misc
Barcode Label 1	72,93	sequence_feature, feature/misc
Barcode Label 4	94,115	sequence_feature, feature/misc
BBa_B0030: Strong RBS	116,130	ribosome_entry_site, feature/rbs
TetR	137,760	feature/protein, CDS
Barcode Label 3	761,782	feature/misc, sequence_feature
Barcode Label 2	783,804	sequence_feature, feature/misc
Barcode Label 5	805,826	sequence_feature, feature/misc
BBa_B0010:T1 (terminator) from E. coli	827,906	sequence_feature, feature/misc
T7 terminator	907,953	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1343005/1

Member of these Collections

• iGEM Parts Registry