Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
Date created: 2014-09-26 11:00:00
Date modified: 2015-05-08 01:09:59
Plac-tetR
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1343006_sequence (Version 1) |
Description
tetR repressor protein under LacI regulated promoterNotes
To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each otherSource
Placeholder| Sequence Annotation | Location | Component / Role(s) |
| LacI Promoter barcode 6 barcode 1 barcode 4 BBa_B0030: Strong RBS tetR barcode 3 barcode 2 barcode 5 BBa_B0010:T1 (terminator) from E. coli T7 terminator | 1,111 112,132 133,153 154,173 174,188 195,822 823,842 843,863 864,884 885,964 965,1016 | promoter feature/promoter feature/barcode engineered_tag feature/barcode engineered_tag feature/barcode engineered_tag feature/rbs ribosome_entry_site CDS feature/protein engineered_tag feature/barcode engineered_tag feature/barcode engineered_tag feature/barcode feature/stop stop_codon stop_codon feature/stop |