BBa_K1343010

BBa_K1343010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1343010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
Date created: 2014-09-26 11:00:00
Date modified: 2015-05-08 01:09:59

PCI-lacI



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1343010_sequence (Version 1)

Description

LacI repressor protein under Lambda phage repressor CI regulated promoter

Notes

To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other

Source

Placeholder

Sequence Annotation Location Component / Role(s)
promoter lamda CI regulator
barcode label 6
barcode label 1
barcode label 4
RBS
space
lacI
barcode label 3
barcode label 2
barcode label 5
Terminator
1,49
50,71
72,93
94,113
114,128
129,134
135,1226
1227,1248
1249,1270
1271,1292
1293,1421
feature/promoter promoter
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
CDS feature/protein
feature/misc sequence_feature
feature/misc sequence_feature
feature/misc sequence_feature
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1343010/1