BBa_K1343014

BBa_K1343014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1343014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon

Date created: 2014-09-26 11:00:00

Date modified: 2015-05-08 01:09:59

Plux-green renilla luciferase

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1343014_sequence (Version 1)

Description

E.coli optimized green renilla luciferase under LuxR-AHL regulated promoter

Notes

To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other

Source

Placeholder

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0062: P lux	1,55	promoter, feature/promoter
barcode 6	56,77	feature/barcode, engineered_tag
barcode 1	78,99	feature/barcode, engineered_tag
barcode 3	100,121	engineered_tag, feature/barcode
BBa_B0030: Strong RBS	122,136	ribosome_entry_site, feature/rbs
green renilla luciferase	143,1078	feature/protein, CDS
barcode 4	1079,1100	engineered_tag, feature/barcode
barcode 2	1101,1122	feature/barcode, engineered_tag
barcode 5	1123,1144	feature/barcode, engineered_tag
BBa_B0010:T1 (terminator) from E. coli	1145,1224	feature/stop, stop_codon
T7 terminator	1225,1271	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1343014/1

Member of these Collections

• iGEM 2018 Distribution Plate 5
• iGEM 2018 Distribution Plate 5 Well 11F
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM 2019 Distribution Plate 5
• iGEM 2019 Distribution Plate 5 Well 12E
• iGEM 2019 Part Library
• iGEM 2019 Plasmid pSB1C3
• iGEM Parts Registry