BBa_K1343018

BBa_K1343018 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1343018
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:09:59

Ptet->tetR->T7



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1343018_sequence (Version 1)

Description

Ptet-tetR. This promoter is induced by aTc. The gene tetR, results in the formation of the repressor tetR and the enzyme T7 polymerase, which induces the promoter PT7. TetR represses the promoter Ptet. It can can be used as a tuner, to repress and control the expression of 7 as a function of the aTc concentration present.
T7 is a very processive enzyme: It causes a very high transcription rate, even resulting in toxicity to the cell. This bio-brick allows us to control the induction of genes under the promoter PT7 without causing toxicity to the cell.

Notes

r

Source

BBa_R0040:TetR repressible promoter, BBa_B0030: Strong RBS, BBa_K145001:T7_polymerase (BL21DE3), BBa_B0010:T1 (terminator) from E. coli.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1343018/1