BBa_K1344004

BBa_K1344004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1344004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: UI Indonesia 2014
Date created: 2014-10-04 11:00:00
Date modified: 2015-05-08 01:10:00

malS-HlyA (with RBS)



Types
DnaRegion

Roles
engineered_region

Translational_Unit

Sequences BBa_K1344004_sequence (Version 1)

Description

This part is based on BBa_K523001, modified by deleting the stop codons. It's also fusion with HlyA secretional tag for extracelullar expression.

Notes

This part makes the alpha-amylase secreted extracelullar

Source

It was modified from BBa_K523001, BBa_K554002

Sequence Annotation Location Component / Role(s)
BglII
Native RBS
malS
ATG
Predicted (cleaved) signal peptide
HlyA
Stop
1,4
20,23
34,2061
34,34
34,84
2070,2249
2250,2255
feature/misc sequence_feature
ribosome_entry_site feature/rbs
CDS feature/cds
start_codon feature/start
feature/misc sequence_feature
CDS feature/cds
feature/stop stop_codon
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1344004/1