BBa_K1351000

BBa_K1351000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1351000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mona Dotzler
Date created: 2014-09-14 11:00:00
Date modified: 2015-06-01 12:53:55

beta-Neurexin-derived peptide binding to S. aureus adhesin SdrC



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1351000_sequence (Version 1)

Description

Short peptide of 11 amino acids corresponding to the N-terminus of the human protein neurexin beta-1. It includes the binding site (HHIHHFH) for the Staphylococcus aureus adhesin SdrC.
Displayed on the surface of Bacillus subtilis, it shoud enable B. subtilis to bind specifically to S. aureus.

Notes

Oligos were designed as precut by EcoRI and PstI to shorten the synthesized fragments and to simplify the cloning into pSB1C3.

Source

The part was generated by annealing of artificially synthesized oligonucletides:
Nrx1b_ENX_SD_Ngo_precutEP_fwd: AATTCgcggccgctTCTAGAgtaaggaggaaGCCGGCATG GCGCACCACATTCACCACTTTCACGGATCA ACCGGTtaatACTAGTagcggccgCTGCA
Nrx1b_SNP_Age_precutEP_rev: GcggccgctACTAGTattaACCGGT TGATCCGTGAAAGTGGTGAATGTGGTGCGC CATGCCGGCttcctccttacTCTAGAagcggccgcG

igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1351000/1