BBa_K1351005

BBa_K1351005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1351005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mona Dotzler

Date created: 2014-10-04 11:00:00

Date modified: 2015-06-01 08:15:22

SigD-dependent flagellin promoter (Phag) of Bacillus subtilis

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1351005_sequence (Version 1)

Description

Phag promoter of B. subtilis flagellin under the control of SigD.

Notes

to be filled

Source

This part was generated by amplification from B. subtilis W168 gDNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.

Phag_ENX_fwd: TGCAGAATTCGCGGCCGCTTCTAGAG ttcacaggaaagaaatttacttgacc
Phag_SNP_rev: AGCCTGCAGCGGCCGCTACTAGTA gaatatgttgttaaggcacgtcc

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-35	121,124	promoter, feature/promoter
-10	140,147	promoter, feature/promoter
transcription start	154,155	feature/start, start_codon

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1351005/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• //regulation
• iGEM 2018 Distribution Plate 5
• iGEM 2018 Distribution Plate 5 Well 2H
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM Parts Registry