comDE

BBa_K1351016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1351016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Judith Heckmann

Date created: 2014-10-01 11:00:00

Date modified: 2015-05-08 01:10:01

comDE Quorum Sensing two component system (Freiburg Standard)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1351016_sequence (Version 1)

Description

Quorum sensing two component system (histidine kinase comD, response regulator comE)of Streptococcus pneumoniae (R6) able to sense the competence stimulating peptide CSP. Part contains optimized RBS and Terminator for Bacillus subtilis.

fwd primer:gatcgaattccgcggccgcttctagataaggaggagccggcATGGATTTATTTGGATTTGGGACGG
rev primer:gatcctgcagcggccgctactagtattaaccggtCTTTTGAGATTTTTTCTCTAAAATATC

Notes

Mutation of two EcoRI and one SpeI recognition sites in comDE

Source

gDNA of Streptococcus pneumoniae R6

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
comD	1,1326	CDS, feature/cds
misc	1323,2072	sequence_feature, feature/misc

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1351016/1

Member of these Collections

• iGEM Parts Registry