BBa_K1351025

BBa_K1351025 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1351025

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Judith Heckmann

Date created: 2014-10-05 11:00:00

Date modified: 2015-06-01 08:13:27

P_comAB, a CSP inducible promoter derived from Streptococcus pneumoniae

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1351025_sequence (Version 1)

Description

CSP-depending promoter of Streptococcus pneumoniae.

This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:

{|
|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:
|GAATTCCGCGGCCGCTTCTAGATAAGGAGGAGCCGGC
|-
|suffix with AgeI, SpeI, NotI and PstI:
|ACCGGTTAATACTAGTAGCGGCCGCTGCAG
|}
Sites of restriction enzymes generating compatible overhangs have the same color:

EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.

Notes

none

Source

gDNA from Streptococcus pneumoniae R6

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ComE binding site Left	62,70	non_covalent_binding_site, feature/binding
ComE binding site Right	83,91	feature/binding, non_covalent_binding_site

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1351025/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• //regulation
• iGEM Parts Registry