BBa_K1351026

BBa_K1351026 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1351026
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mona Dotzler
Date created: 2014-10-04 11:00:00
Date modified: 2015-06-01 12:56:16

Signal peptide of B. subtilis major autolysin LytC



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K1351026_sequence (Version 1)

    Description

    Signal peptide (amino acids 1 to 25) of Bacillus subtilis major autolysin LytC (N-acetylmuramoyl-L-alanine amidase, Uniprot Q02114). Targets proteins for secretion via the Sec pathway (Tjalsma et. al., 2000).

    Notes

    none

    Source

    This part was generated by amplification from BioBrick BBa_K1351006 with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.

    LytC_ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC TTGCGTTCTTATATAAAAGTCCTAACAATGTG

    LytcSP_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT ATCGGCCAAAGCTGTTGGC

    igem#experience
    None
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1351026/1
     

    Attachments

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