| Types || |
| Roles |
| Sequences || BBa_K1351034_sequence (Version 1)|
Description"This part was generated in a modified version of RFC25, and has the following prefix and suffix:
|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:
|suffix with AgeI, SpeI, NotI and PstI:
Sites of restriction enzymes generating compatible overhangs have the same color:
EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined."
NotesThe LMU Bacillus RBS collection is based on a wobble-library with the sequence ARRRRRRGATA and the theoretical translation initiation rates of the RBS in this library, calculated by the salis lab's RBS calculator (https://salis.psu.edu/software/). The 7 RBS submitted as BioBricks are the ones that offer the best coverage of translation initiation rate space, 1 being the optimal Bacillus RBS and 7 being the weakest.