BBa_K1351034

BBa_K1351034 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1351034
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Florian Riemer
Date created: 2014-10-05 11:00:00
Date modified: 2015-06-01 07:44:23

LMU Bacillus RBS collection 7



    • Details

    Types
    DnaRegion

    Roles
    ribosome_entry_site

    RBS

    Sequences BBa_K1351034_sequence (Version 1)

    Description

    "This part was generated in a modified version of RFC25, and has the following prefix and suffix:

    {|
    |prefix with EcoRI, NotI, XbaI, SD and NgoMIV:
    |GAATTCGCGGCCGCTTCTAGATGGCCGGC
    |-
    |suffix with AgeI, SpeI, NotI and PstI:
    |ACCGGTTAATACTAGTAGCGGCCGCTGCAG
    |}
    Sites of restriction enzymes generating compatible overhangs have the same color:

    EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined."

    Notes

    The LMU Bacillus RBS collection is based on a wobble-library with the sequence ARRRRRRGATA and the theoretical translation initiation rates of the RBS in this library, calculated by the salis lab's RBS calculator (https://salis.psu.edu/software/). The 7 RBS submitted as BioBricks are the ones that offer the best coverage of translation initiation rate space, 1 being the optimal Bacillus RBS and 7 being the weakest.

    Source

    Synthetic

    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1351034/1
     

    Attachments

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