BBa_K1351035

BBa_K1351035 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1351035

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Florian Riemer

Date created: 2014-10-05 11:00:00

Date modified: 2015-05-08 01:10:02

α
-helical peptide linker

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K1351035_sequence (Version 1)

Description

This part was generated in a modified version of RFC25, and has the following prefix and suffix:

{|
|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:
|GAATTCGCGGCCGCTTCTAGATGGCCGGC
|-
|suffix with AgeI, SpeI, NotI and PstI:
|ACCGGTTAATACTAGTAGCGGCCGCTGCAG
|}
Sites of restriction enzymes generating compatible overhangs have the same color:

EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined.

Notes

This linker was designed to form a helical secondary structure

Source

Synthetic

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1351035/1

Member of these Collections

• iGEM Parts Registry