Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James Long
Date created: 2014-10-02 11:00:00
Date modified: 2015-05-08 01:10:02
Ice Nucleation Protein (INP) Yellow Florescent Protein (YFP) FLAG-tag
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1352004_sequence (Version 1) |
Description
K1352004 is a part which has a FLAG-tag octapeptide flanked by a multiple cloning site (MCS) inserted into Bba_K523013 (K523013 expresses ice nucleation protein (INP) fused to yellow florescent protein (YFP) by a linker); specifically, on the end of the C-terminus of yellow florescence protein (YFP) and is in the same reading frame as INP+YFP.The FLAG-tag is a BglII restriction site, followed by a FLAG octapeptide, followed by a HindIII restriction site; the sequence of which is as follows; the octapeptide is uppercase, the restriction sites are lowercase:
(agatctGATTATAAAGATGATGATGATAAAaagctt)
Attaching a FLAG-tag to the end of YFP means that it will be expressed on the surface of the cell, the purpose of this is to allow the rapid insertion (via the MCS) of polypeptides (an antigen for example) to the C-terminus end of INP+YFP; this allows the surface expression of said polypeptide.
For the 2014 Aberdeen iGEM project, this part was intended to be used as a trypanosome antigen displayer.
Notes
n/aSource
This part was adapted from BBa_K523013| Sequence Annotation | Location | Component / Role(s) |
| Yellow Florescent Protein Ice Nucleation Protein FLAG Tag | 1607,2323 634,1606 2330,2353 | feature/protein CDS feature/protein CDS tag feature/tag |