BBa_K1356001

BBa_K1356001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1356001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Garrett Jensen
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:02

LMD-9 CRISPR 3 System



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_K1356001_sequence (Version 1)

Description

This is the Type II CRISPR3 system taken from Streptococcus thermophilus LMD-9. It is the Cas9, Csn1, Cas1, and Cas2 proteins along with the tracrRNA but . It may be used with a novel spacer/repeat region to target bacteriophage, plasmids, or any other form of incoming DNA.

Notes

This part has many restriction sites present. We have removed 3 EcoRI sites and 2 SpeI sites from this CRISPR so that it can be used with the iGEM plasmid.

Source

Streptococcus thermophilus LMD-9 genomic DNA.

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1356001/1