Types | DnaRegion
|
Roles | DNA
sequence_feature
|
Sequences | BBa_K1356001_sequence (Version 1)
|
Description
This is the Type II CRISPR3 system taken from Streptococcus thermophilus LMD-9. It is the Cas9, Csn1, Cas1, and Cas2 proteins along with the tracrRNA but . It may be used with a novel spacer/repeat region to target bacteriophage, plasmids, or any other form of incoming DNA.
Notes
This part has many restriction sites present. We have removed 3 EcoRI sites and 2 SpeI sites from this CRISPR so that it can be used with the iGEM plasmid.
Source
Streptococcus thermophilus LMD-9 genomic DNA.