BBa_K136005

BBa_K136005 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K136005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cyprien Maisonnier
Date created: 2008-08-27 11:00:00
Date modified: 2015-05-08 01:10:02

FlhDC gene from



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K136005_sequence (Version 1)

Description

FlhDC is the master regulator of the flagella construction system.

This hexameric transcription factor (FlhD4C2
The expression of FlhDC is regulated by several transcription factors such as OmpR and EnvZ.

Notes

The primers used to amplify this sequence are :

*Forward :

GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTA
*Reverse :

GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAACAGCCTGTACTCTCTGTTCATCC

Source

This part is from E. coli" K 12 strain MG1655 genome.

Sequence Annotation Location Component / Role(s)
Natural RBS of FlhDC
Start codon
FlhD coding sequence
Stop codon
FlhC coding sequence
Start codon
Stop codon
1,39
40,42
40,390
388,390
393,974
393,395
969,974
feature/rbs ribosome_entry_site
feature/start start_codon
feature/protein CDS
stop_codon feature/stop
CDS feature/protein
start_codon feature/start
feature/stop stop_codon
igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K136005/1