Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Beno??t D
Date created: 2008-10-28 12:00:00
Date modified: 2015-05-08 01:10:03
fliL promoter + WT RBS ( K136009) - EnvZ*
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K136064_sequence (Version 1) |
Description
EnvZ is a transmembrane histidine kinase that, in response to osmotic changes, phosphorylates OmpR the natural inhibitor of flhDC promotor the master regulator of the flagella synthesis. EnvZ* is here a mutated version which constitutively phosphorylates OmpR.fliL is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon, ).
Its promoter is consitutively repressed.
It is activated by flhD4C2 and by fliA with different forces ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).
This construction may allow ,with the add of a reporter tag, to see some oscillations
Notes
Assembled biobricksSource
the two parts of this construction were obtained by cloning from the genome of MG1655 WT and mutated for EnvZ bacteria strain| Access | Instance | Definition |
| public public | BBa_K136009 BBa_K136046 | BBa_K136009 BBa_K136046 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K136009 BBa_K136046 | 1,154 161,1513 | BBa_K136009 BBa_K136046 |