BBa_K136065

BBa_K136065 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K136065
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Beno??t D
Date created: 2008-10-28 12:00:00
Date modified: 2015-05-08 01:10:03

flhB promoter + WT RBS - GFP



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K136065_sequence (Version 1)

Description

GFP under the control of flhB, a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ).

Its promoter is consitutively repressed.

It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).

Notes

assembled biobricks

Source

for the promoter cloning from the genome of MG1655 WT bacteria strain

Access Instance Definition
public
public
BBa_K136008
BBa_E0040
BBa_K136008
GFP
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K136065/1