BBa_K136065

BBa_K136065 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K136065

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Beno??t D

Date created: 2008-10-28 12:00:00

Date modified: 2015-05-08 01:10:03

flhB promoter + WT RBS - GFP

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K136065_sequence (Version 1)

Description

GFP under the control of flhB, a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ).

Its promoter is consitutively repressed.

It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).

Notes

assembled biobricks

Source

for the promoter cloning from the genome of MG1655 WT bacteria strain

Components

Access	Instance	Definition
public	BBa_K136008	BBa_K136008
public	BBa_E0040	GFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K136008	1,203	BBa_K136008
BBa_E0040	210,929	GFP

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K136065/1

Member of these Collections

• iGEM Parts Registry