BBa_K1361995

BBa_K1361995 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1361995

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shoujie Sun

Date created: 2014-10-03 11:00:00

Date modified: 2015-05-08 01:10:04

CsgBtrunc, curli fiber nucleator with a C-terminnal deletion

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1361995_sequence (Version 1)

Description

This part is Curli fiber nucleator subunit protein CsgB with deletion of C-terminal amino acid from 133 to 155 that is lost the ability to attach outer membrane of the cell.

Notes

Deletion is designed referring Hammer, Neal D., Jens C. Schmidt, and Matthew R. Chapman. "The curli nucleator protein, CsgB, contains an amyloidogenic domain that directs CsgA polymerization." Proceedings of the National Academy of Sciences 104.30 (2007): 12494-12499.

Source

This part is collected by PCR from genome of E coli. DH5alpha.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cds	1,399	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1361995/1

Member of these Collections

• iGEM Parts Registry