BBa_K1361998

BBa_K1361998 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1361998

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shoujie Sun

Date created: 2014-10-02 11:00:00

Date modified: 2015-05-08 01:10:04

Curli Fiber assembly associated protein CsgA, CsgC

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1361998_sequence (Version 1)

Description

CsgA, CsgC genes are directly PCRed from E coli. DH5alpha genome as a whole. There is a scar sequence between CsgA and CsgC coding region that contains a promoter sequence may constitutively transcript CsgC gene.
CsgA protein is the major subunit of Curli Fiber. CsgC may regulate CsgG outer membrane assembly of channel protein CsgG and pore activity through modification of C230 in CsgG.

Notes

The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.

Source

PCR on genomic DNA from E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
CsgA	1,456	feature/cds, CDS
start	1,3	feature/start, start_codon
stop	454,456	feature/stop, stop_codon
CsgC	515,847	feature/cds, CDS
start	515,517	start_codon, feature/start
stop	845,847	stop_codon, feature/stop

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1361998/1

Member of these Collections

• iGEM Parts Registry