T7 RBS

BBa_K1362090 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1362090
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
Date created: 2014-10-02 11:00:00
Date modified: 2015-05-08 01:10:04

strong T7 RBS



Types
DnaRegion

Roles
ribosome_entry_site

RBS

Sequences BBa_K1362090_sequence (Version 1)

Description

RFC10 compatible strong RBS derived from the T7 phage gene 10a (major capsid protein)[1]. When assembled to a coding part with the A of the start codon being part of the XbaI site, the RBS will be shifted one bp downstream compared to the native sequence.

The sequence was successfully used by the iGEM team Heidelberg 2014 for the expression of many proteins in E.coli.


1. Olinss, P. & Rangwala, S. H. Derived from Bacteriophage T7 mRNA Acts ELS an Enhancer of Translation of the lac2 Gene in. 16973???16976 (1989).

Notes

The 18 bp including the XbaI that can be found upstream of the presumably important part of the RBS were included into the sequence just to make sure it works. However to fully comply with RFC10 a G was inserted behind the XbaI site.

Source

synthesized as found in the T7 genome and several commercial expression plasmids.

Sequence Annotation Location Component / Role(s)
t7 RBS
Shine-Dalgarno
11,28
21,28
ribosome_entry_site feature/rbs
feature/misc sequence_feature
igem#experience Works  
igem#sampleStatus It's complicated  
igem#status Planning  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K1362090/1