Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:10:05
mutated (c-terminal Ser ->Ala) NpuDnaE C-Intein cloning piece
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1362399_sequence (Version 1) |
Description
This is a mutated version of the NpuDnaE C-intein. The C-terminal Histidine was mutated to an Alanine to prevent protein splicing. This part therefore usually serves as an experimental control. See all other specifications to this part inNotes
Design of a control construct for various assays.Source
See| Sequence Annotation | Location | Component / Role(s) |
| NpuDnaE C-Intein to prevent formation of SpeI site | 1,101 99,99 | feature/protein CDS sequence_alteration feature/mutation |