RFC105 NN

BBa_K1362415 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1362415
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:05

N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN



Types
DnaRegion

Roles
polypeptide_domain

Protein_Domain

Sequences BBa_K1362415_sequence (Version 1)

Description

This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [page] as well as in RFC[???]. Specifically, this part contains the overhang A used to insert a protein behind any RFC[10] compatible RBS and in-frame with the start-codon. It lies within the four bases formed by the second last base of the XbaI/SpeI scar or the first base in front of start codon respectively and the start codon itself.

Notes

This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.

Source

This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References[1]]]. All standard sequences can be reviewed in RFC[???] [[#References[2]]].

igem#experience None  
igem#sampleStatus Not in stock  
igem#status Unavailable  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K1362415/1