Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:05
C-terminal GG-linker overhang GGTGGT=Gly+Gly RFC[105] overhang NC
| Types | DnaRegion |
| Roles | Protein_Domain polypeptide_domain |
| Sequences | BBa_K1362417_sequence (Version 1) |
Description
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's wiki page as well as in RFC[???]. Specifically, this part contains the overhang C used to insert a protein-tag or similar in front of the C-terminal end of a N-Intein. It lies within the a double-glycine linker piece.Notes
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.Source
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All standard sequences can be reviewed in RFC[???] [[#References|[2]]].| Sequence Annotation | Location | Component / Role(s) |
| RFC[???] C GlyGly | 3,6 1,6 | conserved_region feature/conserved feature/scar |