| Types | DnaRegion
|
| Roles | polypeptide_domain
Protein_Domain
|
| Sequences | BBa_K1362420_sequence (Version 1)
|
Description
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's
wiki page as well as in RFC[???]. Specifically, this part contains the overhang E* used to insert a protein in front of a non-splicing N-Intein, which could often serve as control for protein splicing experiments. It lies within the four bases formed by the glycine and the first base of the subsequent Leucine/Isoleucine or similar of the mutated N-terminal splicing site.
Notes
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
Source
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All standard sequences can be reviewed in RFC[???] [[#References|[2]]].
