| Types || |
| Roles |
| Sequences || BBa_K1362421_sequence (Version 1)|
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's wiki page
as well as in RFC[???]. Specifically, this part contains the overhang F used to insert the last part of an intein-fused protein directly in front of an RFC double stop-codon. It lies within the first for bases of the stopstop sequence.
NotesThis part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
SourceThis standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|]]. All standard sequences can be reviewed in RFC[???] [[#References|]].