BsaI ->

BBa_K1362424 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1362424

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha

Date created: 2014-10-06 11:00:00

Date modified: 2015-05-08 01:10:05

BsaI restriction site for RFC[105] cloning

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_K1362424_sequence (Version 1)

Description

This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible port-translational modifications.

Notes

This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.

Source

This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[???]

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K1362424/1

Member of these Collections

• iGEM Parts Registry