Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:10:05
BsaI restriction site for RFC[???] cloning (in part in prefix)
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K1362425_sequence (Version 1) |
Description
This Sequence starts with a part of the BsaI recognition site. The missing G of the recognition site can be found in the upstream BB prefix. It contains a spacer nucleotide so that BsaI will cut the top strand directly downstream and the bottom strand 4 nucleotides downstream.| Sequence Annotation | Location | Component / Role(s) |
| BsaI site RFC[???] standard overhang A | 1,5 5,5 | feature/misc sequence_feature feature/misc sequence_feature |