TEV clv.

BBa_K1362453 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1362453

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha

Date created: 2014-10-07 11:00:00

Date modified: 2015-05-08 01:10:06

TEV protease recognition/cleavage site

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K1362453_sequence (Version 1)

Description

This part codes for the amino acid sequence ENLYFQG. This site is recognized by TEV protease (catalytic domain of the Tobacco Etch Virus nuclear inclusion a (NIa) protein), which will cleave the Gln-Gly peptide bond [[{{PAGENAME}}:Design#References|[1]]]. The final four nucleotides of this sequence are GGGT, which will be the overhang produced wenn a reverse-facing BsaI site (BBa_K1362423, BBa_K1362427, BBa_K1362447) is directly following this part, as in the Sortase A circularization constructs (BBa_K1362202, BBa_K1362203, BBa_K1362204, BBa_K1362205).

Source

Tobacco Etch Virus

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1362453/1

Member of these Collections

• iGEM Parts Registry