SortA clv.

BBa_K1362454 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1362454
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:10:06

Sortase A recognition/cleavage site



    • Details

    Types
    DnaRegion

    Roles
    polypeptide_domain

    Protein_Domain

    Sequences BBa_K1362454_sequence (Version 1)

    Description

    This part codes for the amino acid sequence LPETGG. This site is recognized by Sortase A, which will cleave the Thr-Gly peptide bond and subsequently act as a transpeptidase to attach any peptide with an N-terminal glycine [[{{PAGENAME}}:Design#References|[1]]]. The first four nucleotides of this sequence are CTTC, which will be the overhang produced wenn a forward-facing BsaI site (BBa_K1362424, BBa_K1362425) is directly following this part, as in the Sortase A circularization constructs (BBa_K1362202, BBa_K1362203, BBa_K1362204, BBa_K1362205).

    Source

    'Staphylococcus aureus

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1362454/1
     

    Attachments

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