BBa_K1362992

BBa_K1362992 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1362992

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jakob Kreft

Date created: 2014-10-04 11:00:00

Date modified: 2015-05-08 01:10:06

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region

Description

Npu DnaE C-Intein protein assembly construct for cloning according to RFC[???]. With N-terminal hexahistidine-tag.

Notes

Part was designed to be used with to RFC[???]. Parts were amplified from source plasmids with matching overhangs and assembled via CPEC.

Source

Npu DnaE C-Intein cloned from pVS41 obtained from Prof. Henning D. Mootz, University of Muenster. BBa_J04450 was used as mRFP selection marker.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
T7 RBS	1,29	feature/rbs, ribosome_entry_site
start	36,38	start_codon, feature/start
His6-tag	39,56	feature/tag, tag
triple-gly linker	57,65	sequence_feature, feature/misc
Npu DnaE C-Intein	66,101	feature/protein, CDS
CAAC overhang	102,106	sequence_feature, feature/misc
BsaI restriction site	108,114	feature/misc, sequence_feature
TAAT overhang	1254,1257	feature/misc, sequence_feature
mRFP selection marker	115,1246	feature/conserved, conserved_region
BsaI restriction site	1248,1253	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: No part sequence
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1362992/1

Member of these Collections

• iGEM Parts Registry